Over 20 Years of Experience in Mushroom Cultivation Solutions.
Preparation of Shiitake Mushroom Mother Culture
(1) Formula
① 200g potato, 20g glucose, 5g peptone, 18-20g agar, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, pH 6.5-7.0, 1000mL water.
② 200g potato, 20g glucose, 50g wheat bran, 18-20g agar, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, pH 6.5-7.0, 1000mL water.
(2) Inoculation and Cultivation
Prepared, sterilized, and inoculated according to standard procedures, incubated at 25℃ in the dark.
(3) Labeling and Cultivation: Before removing the test tubes from the inoculation box, label each tube with the strain number and inoculation date. Then, place the tubes in an incubator (room) at 23–25°C for 8–10 days until the culture slant is fully grown.
(4) Strain Preservation: Select uncontaminated culture slants, wrap them in paper, and store them in a refrigerator at 4°C.
Henan Joconn Machinery Co., Ltd. is an enterprise specializing in the production of mushroom equipment. It is engaged in the development, production and application of machinery and equipment for mushroom growth production lines.