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Sterilization and cooling operation steps of the culture medium of Flammulina velutipes
Sterilization and cooling operation steps of the culture medium of Flammulina velutipes
After the culture bottles (bags) are filled, they are placed in the turnover basket and placed on the sterilization cart, and pushed into the sterilizer for sterilization. The key issue for the success of factory cultivation is that the sterilization must be thorough, otherwise everything is in vain. Sterilization containers include normal pressure sterilizers, high pressure sterilizers, and vacuum high pressure sterilizers. Factory production generally uses high pressure sterilizers and vacuum sterilizers.
(I) Sterilization
Sterilization requires sufficient time and thoroughness. The following uses the vacuum sterilizer (9600 bottles per pot) as an example to introduce the key points of sterilization for your reference.
1. Sterilization procedure
Check the sterilization procedure → confirm each valve and switch → push the cart into the sterilization cabinet → confirm the quantity → close the cabinet door → confirm the cabinet door sealing condition → start the sterilization procedure → record → confirm the temperature change.
2. Specific sterilization operation requirements
① Open the cabinet door and confirm that the doors at both ends cannot be opened at the same time.
② After the bottles are loaded, slowly push the sterilization cart into the sterilization cabinet and place them neatly from the inside to the outside.
③ Confirm that 24 cargo carts (25 per cart, 16 bottles per cart) are placed in the sterilization cabinet and then close the sterilization valve.
④ Confirm that the cabinet door is completely closed and the sealing is intact.
⑤ Confirm that the setting value of the sterilization program is a sterilization pressure of 0.15MPa, 105℃ insulation for 30min, 110℃ insulation for 30min, an effective sterilization temperature of 122℃, insulation for 80min, and 20min.
⑥ Start the automatic sterilization program and confirm the temperature rise in the sterilization cabinet.
⑦ The temperature rise in the sterilization cabinet is 100℃, and confirm that the pressure in the cabinet is positive. Before maintaining the pressure, the cold air in the pot should be exhausted. When the pressure rises from 0MPa to 0.05MPa, start to discharge the cold air to reduce the pressure to 0MPa. Then increase the pressure to 0.1MPa, and then discharge the air to reduce the pressure to 0MPa. The number of times of discharging air shall not be less than 2 times.
⑧Confirm that the temperature in the sterilizer is 105℃ and keep it warm for 30 minutes.
⑨Automatically run the sterilization program and the temperature rises to 122℃, keep it warm for 80 minutes, and leave it in a stale state for 20 minutes.
⑩Automatically run the sterilization program until the end, and keep relevant records.
①Fully confirm the operation of the sterilizer and do a good job of environmental sanitation cleaning
3. Sterilization precautions
1. The quantity and density in the sterilizer should be placed according to the regulations. If the quantity is too large or the density is too high, the steam penetration will be affected and the sterilization time will be relatively extended.
2. If the formula of the culture medium changes, the space between the substrates may become smaller or larger, and the sterilization procedure must be modified accordingly, otherwise it may cause pollution or waste of energy.
Henan Joconn Machinery Co., Ltd. is an enterprise specializing in the production of mushroom equipment. It is engaged in the development, production and application of machinery and equipment for mushroom growth production lines.