What should be paid attention to when inoculating solid and liquid spawn of king oyster / Pleurotus eryngii?

What should be paid attention to when inoculating solid and liquid spawn of king oyster /Pleurotus eryngii?

1. Inoculation with solid spawn
When the temperature in the material cools down to below 25℃, start inoculation to reduce the chance of infection by competitive bacteria. In production, the disinfection and sterilization of the inoculation room is turned on 4~5h before inoculation, and the air purification system is turned on 1h before the equipment is inoculated to ensure that the inoculation room is in a positive pressure state during the entire inoculation process.

Inoculation staff must wear disinfected inoculation clothing, shoes and hats to enter the inoculation room, wipe their hands with 75% alcohol cotton balls for disinfection, wipe the inoculation tools with 75% alcohol for disinfection and cool them by flame burning before use.

The spawn used on the day must be strictly selected by a dedicated person for pre-disinfection. When using, dig out the 1~2cm old mushroom skin on the surface, and pour the crushed spawn into the hole of the material bag with inoculation to ensure that the spawn can fall directly into the bottom of the material hole to speed up the speed of filling the mycelium bag and ensure that the mycelium age is consistent at the top and bottom. The inoculation amount standard is to fill the reserved holes and evenly cover the material surface with 0.3cm thick spawn.

2. Inoculation with liquid strains

(1) Requirements for purification workshops

The purification workshop is required to install a central air filtration system, a refrigeration system and an ozone disinfection system;

The rooms include the first cooling room, the strong cooling room, the inoculation room, the inoculation room and the strain processing room. The workshop entrance must have a buffer room and a disinfection pool, and the inoculation room must be equipped with a 100-level air filtration system and a mechanical water conveyor line.

(2) Inoculation

Before inoculation, the cooling process of the culture bottles (bags) must be included. All air filtration and disinfection systems must be operated strictly in accordance with the startup procedures, and the entry and exit of people must also be strictly in accordance with the purification workshop personnel entry and exit disinfection procedures.

During inoculation, the culture bottles (bags) and baskets cooled to below 25°C are moved from the sterilization trolley to the dedicated inoculation line. Under the air high-efficiency filter in the inoculation room, the staff use an automatic liquid inoculator to spray the fermented liquid strains on the surface of the bottle (bag) mouth under the pressure tank 0.20MPa. Each bottle (bag) is inoculated with 25~30ml.

The inoculation environment must be absolutely clean and controlled at the 10,000-grade level. For specific operations, please refer to the section on inoculation of liquid fungus of Flammulina velutipes. After inoculation, the whole basket of cultivation bottles (bags) is transported from the assembly line to the outdoors, and then moved to the culture room for cultivation by a dedicated person using a turnover vehicle.